Electrooptics Studies of Escherichia coli Electropulsation: Orientation, Permeabilization, and Gene Transfer
Identifieur interne : 003B73 ( Main/Exploration ); précédent : 003B72; suivant : 003B74Electrooptics Studies of Escherichia coli Electropulsation: Orientation, Permeabilization, and Gene Transfer
Auteurs : N. Eynard [France] ; F. Rodriguez [France] ; J. Trotard [France] ; J. Teissié [France]Source :
- Biophysical Journal [ 0006-3495 ] ; 1998.
English descriptors
- Teeft :
- Axial ratio, Bacteria, Bacterial population, Bacterial suspension, Biophys, Biophysical journal figure, Biophysical journal volume, Blood cells, Cell content, Cell envelope, Cell membrane, Cell membranes, Cell permeabilization, Cell surface, Cell suspension, Coli, Coli permeabilization, Colloid interface, Critical step, Cytoplasmic leakage, Dipole effect, Direct observation, Dual approach, Effective permeabilization, Electric birefringence, Electric field, Electric fields, Electric pulse, Electrical breakdown, Electrical field, Electrooptical method, Electrooptics, Electropermeabilization, Electroporation, Electropulsation, Electrotransformation, Envelope alteration, Experimental observation, External field, Eynard, Field amplitude, Field direction, Field effect, Field intensity, Field lines, Field strength, Field strengths, Form factor, Gene transfer, Imax, Imax increases, Incident beam, Infinite values, Intact yeast cells, Ionic content, Kakorin, Kerr effect, Kinetic scheme, Kinetic studies, Kinetic study, Kinetics, Leakage, Light data, Light decrease, Linear adjustments, Linear function, Lipid vesicles, Long axes, Long axis, Major part, Mammalian cells, Membrane, Membrane electroporation, Microsecond pulses, Millisecond time range, Multistep processes, Neumann, Orientation, Orientation phenomenon, Orientation process, Output signal, Permeabilization, Permeabilization process, Permeabilization processes, Permeabilized, Plasmid, Plasmid addition, Plateau value, Potential difference, Present study, Present work, Previous work, Pulse, Pulse duration, Rate constants, Refractive index, Single pulse, Small molecules, Spherical cells, Stainless steel, Teissie, Theoretical interpretation, Theoretical prediction, Time courses, Tobacco mosaic virus, Toulouse cedex, Tsong, Turbidity, Turbidity signal, Vesicle, Video, Video method, Video microscopy studies, Video monitoring.
Abstract
Abstract: Fast optical transient signals are suitable approaches to the investigation of the behavior of bacteria during an electric pulse. In a previous work, by a dual approach taking advantage of a video method and a fast kinetic study of the light transmitted across a cell suspension, we showed that a field-induced orientation phenomenon was affecting the rod-shaped bacteria during the pulse (Eynard et al., 1992. Eur. J. Biochem. 209:431–436). In the present work, time courses of electro-induced responses of bacteria during a single square-wave pulse are analyzed. Observations of both the orientation step and the permeabilization process are relevant. These two steps are affected by the addition of DNA. They both obey to a first-order kinetic. The conclusion of this work is that Escherichia coli permeabilization and transformation are multistep processes: orientation (step 1) is followed by an envelope alteration (step 2), all steps being affected by plasmid addition. In the case of E. coli, a rod-shaped bacteria, the orientation process (step 1) brings the cell parallel to the field direction. The pulse duration must be longer than the orientation characteristic time (≈ 1ms) to trigger an effective permeabilization and its associated events. The permeabilization process (step 2) is associated with a field-induced dipole effect.
Url:
DOI: 10.1016/S0006-3495(98)77704-5
Affiliations:
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In a previous work, by a dual approach taking advantage of a video method and a fast kinetic study of the light transmitted across a cell suspension, we showed that a field-induced orientation phenomenon was affecting the rod-shaped bacteria during the pulse (Eynard et al., 1992. Eur. J. Biochem. 209:431–436). In the present work, time courses of electro-induced responses of bacteria during a single square-wave pulse are analyzed. Observations of both the orientation step and the permeabilization process are relevant. These two steps are affected by the addition of DNA. They both obey to a first-order kinetic. The conclusion of this work is that Escherichia coli permeabilization and transformation are multistep processes: orientation (step 1) is followed by an envelope alteration (step 2), all steps being affected by plasmid addition. In the case of E. coli, a rod-shaped bacteria, the orientation process (step 1) brings the cell parallel to the field direction. The pulse duration must be longer than the orientation characteristic time (≈ 1ms) to trigger an effective permeabilization and its associated events. The permeabilization process (step 2) is associated with a field-induced dipole effect.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Midi-Pyrénées</li><li>Occitanie (région administrative)</li></region><settlement><li>Toulouse</li></settlement></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Eynard, N" sort="Eynard, N" uniqKey="Eynard N" first="N." last="Eynard">N. Eynard</name></region><name sortKey="Rodriguez, F" sort="Rodriguez, F" uniqKey="Rodriguez F" first="F." last="Rodriguez">F. Rodriguez</name><name sortKey="Teissie, J" sort="Teissie, J" uniqKey="Teissie J" first="J." last="Teissié">J. Teissié</name><name sortKey="Teissie, J" sort="Teissie, J" uniqKey="Teissie J" first="J." last="Teissié">J. Teissié</name><name sortKey="Trotard, J" sort="Trotard, J" uniqKey="Trotard J" first="J." last="Trotard">J. Trotard</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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